|
The following table
contains an alphabetical listing of commonly used single-dye
applications. Filter sets for multiple dye applications can be found
by looking under the heading on the filter sets page.
If you cannot find your
particular fluorophore in this listing, please contact as
we will be able to advise you of the most suitable filters to use.
Click on any of the
recommended filter set numbers for a component breakdown and spectra.
|
|
|
|
|
|
|
|
Fluorophore |
Ex |
Em |
Alpha
Vivid |
Vivid
Classic |
Vivid
Plus |
Vivid
Standard |
Other |
Acridine
Orange (+DNA) |
500 |
526 |
104,105 |
|
|
|
|
Acridine
Organe (+RNA) |
460 |
650 |
|
|
19 |
21 |
|
Alexa
Fluor® 350 |
347 |
442 |
|
|
02,05 |
03,06 |
|
Alexa
Fluor® 430 |
434 |
540 |
|
15 |
|
14 |
|
Alexa
Fluor® 488 |
495 |
519 |
100,115 |
25 |
|
22,23 |
|
Alexa
Fluor® 532 |
531 |
554 |
104,105 |
|
30 |
99 |
|
Alexa
Fluor® 546 |
556 |
573 |
101,
108 |
38,34 |
|
32,37 |
|
Alexa
Fluor® 568 |
579 |
604 |
102 |
|
40 |
41 |
|
Alexa
Fluor® 594 |
591 |
618 |
102 |
|
40 |
41,
42,
43 |
|
Alexa
Fluor® 633 |
632 |
647 |
|
|
|
|
|
Alexa
Fluor® 660 |
663 |
690 |
|
|
|
|
|
Alexa
Fluor® 680 |
679 |
702 |
|
|
|
|
|
Allophycocyanin
(APC) |
650 |
660 |
110 |
|
|
44,
45,
70 |
|
AMCA/AMCA-X |
345 |
445 |
|
|
02,
05 |
03,
06 |
|
7-Aminoactinomycin
D (7-AAD) |
546 |
647 |
103 |
34,
38 |
|
35,
36 |
|
7- Amino-4-methylcoumarin |
351 |
430 |
|
|
02,
05 |
03,
06 |
|
6-Aminoquinoline |
355 |
550 |
|
08 |
02,
05 |
09 |
|
Aniline Blue |
370 |
509 |
|
08 |
13 |
09 |
|
ANS |
372 |
455 |
|
|
02,
05 |
|
|
ATTO-TAG
CBQCA |
465 |
560 |
|
|
18,
19 |
|
|
ATTO-TAG
FQ |
486 |
591 |
103 |
|
|
21 |
|
Auramine O-Feulgen |
460 |
550 |
|
|
19 |
20,
71 |
|
BCECF (high pH) |
503 |
528 |
|
|
|
|
16 |
BFP (Blue
Fluorescent Protein) |
382 |
448 |
129 |
|
|
|
|
BFP/GFP FRET |
382 |
508 |
|
|
|
|
89 |
BOBO-1,
BO-PRO-1 |
462 |
481 |
114,
130 |
|
|
|
|
BOBO-3,
BO-PRO-3 |
570 |
604 |
102 |
|
40 |
41,
42 |
|
BODIPY® FL |
505 |
513 |
100,
115 |
25 |
|
22,
23 |
|
BODIPY® TMR |
542 |
574 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
BODIPY® TR-X |
589 |
617 |
102 |
|
40,
43 |
41,
42 |
|
BODIPY® 530/550 |
533 |
550 |
101,
108 |
30 |
|
|
|
BODIPY® 558/568 |
558 |
568 |
101,
108 |
34 |
|
32,
33 |
|
BODIPY® 564/570 |
564 |
570 |
101,
108 |
34,
38 |
|
32,
33 |
|
BODIPY® 581/591 |
582 |
590 |
102 |
|
40 |
39 |
|
BODIPY® 630/650-X |
630 |
650 |
|
|
|
45,
46 |
|
BODIPY® 650-665-X |
650 |
665 |
110 |
|
|
47,
70 |
|
BTC |
401/464 |
529 |
|
|
|
|
133 |
Calcein |
494 |
517 |
100,
115 |
25 |
|
22,
23 |
|
Calcein Blue |
375 |
420 |
|
|
02,
05 |
03,
06 |
|
Calcium Crimson |
590 |
615 |
102 |
|
40 |
41,
42,
43 |
|
Calcium Green-1 |
506 |
531 |
100,
115 |
25 |
|
22,
23 |
|
Calcium Orange |
549 |
576 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Calcofluor®
White |
350 |
440 |
|
|
02,
05 |
03,
06 |
|
5-Carboxyfluoroscein
(5-FAM) |
492 |
518 |
100,
115 |
25 |
|
22,
23 |
|
5-Carboxynaphthofluoroscein |
598 |
668 |
102 |
|
|
44,
45 |
|
6-Carboxyrhodamine
6G |
525 |
555 |
104,
105 |
30 |
|
|
|
5-Carboxytetramethylrhodamine
(5-TAMRA) |
542 |
568 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Carboxy-X-rhodamine
(5-ROX) |
574 |
602 |
102 |
|
40 |
39,
41 |
|
Cascade Blue® |
400 |
420 |
|
|
13 |
10,
11 |
|
Cascade Yellow |
402 |
545 |
|
|
13 |
14,
76 |
|
CCF2 (GeneBLAzer) |
402 |
520 |
|
|
106 |
|
124 |
CFP (Cyan
Fluorescent Protein) |
458 |
480 |
114,
130 |
|
|
|
|
CFP/YFP FRET |
458 |
527 |
|
|
|
|
88 |
Chromomycin A3 |
450 |
470 |
114,
130 |
|
13,
78 |
|
|
Cl-NERF
(low pH) |
504 |
540 |
104,
105 |
|
30 |
|
16 |
CPM |
385 |
471 |
|
|
|
10,
11 |
|
6-CR 6G |
518 |
543 |
104,
105 |
30 |
|
|
|
CTC Formazan |
450 |
630 |
|
|
|
21 |
|
Cy2® |
489 |
506 |
100,
115 |
25 |
|
22,
23 |
|
Cy3® |
550 |
570 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Cy3.5® |
581 |
596 |
102 |
|
40 |
41 |
|
Cy5® |
649 |
670 |
110 |
|
|
45,
46,
47, 70 |
|
Cy5.5® |
675 |
694 |
|
|
48 |
|
|
Cy7® |
743 |
767 |
143
|
|
|
|
|
Dansylamine |
340 |
525 |
|
08 |
|
09 |
|
Dansyl cadaverine |
335 |
518 |
|
08 |
02,
05 |
|
|
Dansylchloride |
380 |
475 |
|
|
13 |
10 |
|
DAPI |
358 |
461 |
|
|
02,
05 |
03,
06 |
|
Dapoxyl |
373 |
574 |
|
08 |
05 |
|
|
DiA (4-Di-16-ASP) |
491 |
613 |
115 |
|
18 |
21 |
|
DiD (DilC18(5)) |
644 |
665 |
100 |
|
|
45,
47, 70 |
|
DIDS |
341 |
414 |
|
|
02 |
03 |
|
Dil (DilC18(3)) |
549 |
565 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
DiO (DiOC18(3)) |
484 |
501 |
100,
115 |
25 |
|
22,
23 |
|
DiR (DilC18(7)) |
750 |
779 |
143 |
|
|
|
|
Di-4 ANEPPS |
488 |
605 |
|
|
|
21 |
17 |
Di-8 ANEPPS |
468 |
635 |
|
|
|
21 |
17 |
DM-NERF
(4.5-6.5 pH) |
510 |
536 |
104,
105 |
30 |
16 |
|
|
DsRed (Red
Fluorescent Protein) |
558 |
583 |
111 |
|
|
|
|
ELF®
-97 alcohol |
345 |
530 |
|
08 |
02,
05 |
09 |
|
Eosin |
524 |
544 |
104,
105 |
30 |
|
|
|
Erythrosin |
529 |
554 |
104,
105 |
30 |
|
|
|
Ethidium bromide |
518 |
605 |
|
34,
38 |
|
35,103 |
28 |
Ethidium
homodimer-1 (EthD-1) |
528 |
617 |
|
34,
38 |
|
35,103 |
28 |
Europium
(III) Chloride |
337 |
613 |
|
08 |
02 |
|
|
5-FAM (5-Carboxyfluorescein |
492 |
518 |
100,
115 |
25 |
|
22,
23 |
|
Fast Blue |
365 |
420 |
|
|
02,
05 |
03,
06 |
|
Fluorescein (FITC) |
494 |
518 |
100,
115 |
25 |
|
22,
23 |
|
Fluo-3 |
506 |
526 |
104,
105 |
|
|
|
|
Fluo-4 |
494 |
516 |
100,
115 |
|
|
|
|
FluorX® |
494 |
519 |
100,
115 |
25 |
|
22,
23 |
|
Fluoro-Gold
(high pH) |
368 |
565 |
|
08 |
02,
05 |
09 |
|
Fluoro-Gold
(low pH) |
323 |
408 |
|
|
02,
05 |
|
|
Fluoro-Jade |
425 |
510 |
|
15 |
|
14 |
|
FM® 1-43 |
479 |
598 |
115 |
25 |
|
|
|
Fura-2
(high calcium) |
335 |
505 |
|
|
|
|
04 |
Fura-2/BCECF |
335/503 |
505/528 |
|
|
|
|
79 |
Fura
Red (high calcium) |
436 |
637 |
|
|
18 |
|
17 |
Fura Red/Fluo-3 |
472/506 |
672/527 |
105 |
|
|
|
26 |
GeneBLAzer
(CCF2) |
402 |
520 |
|
|
106 |
|
124 |
GFP Red
Shifted (rsGFP) |
488 |
508 |
100,
115,
116 |
|
|
|
|
GFP Wild
Type, UV Excitation |
395 |
508 |
|
|
|
|
76 |
GFP Wild
Type, non-UV Excitation |
470 |
508 |
100,
115 |
|
|
|
|
GFP/BFP FRET |
382 |
508 |
|
|
|
|
89 |
GFP/DsRed FRET |
470 |
585 |
|
|
|
|
|
Hoechst
33342 & 33258 |
352 |
461 |
|
|
02,
05 |
03,
06 |
|
7-Hydroxy-4-methylcoumarin
(pH 9) |
360 |
449 |
|
|
02,
05 |
03,
06 |
|
1,5 IAEDANS |
336 |
482 |
|
|
02 |
|
|
Indo-1
(high calcium) |
330 |
401 |
|
|
|
|
07 |
Indodicarbocyanine |
644 |
665 |
110 |
|
|
45,
46,
47, 70 |
|
Indotricarbocyanine |
739 |
765 |
143-2 |
|
|
|
|
JC-1 |
498/593 |
525/595 |
115 |
|
|
26 |
|
6-JOE |
525 |
555 |
104,
105 |
30 |
|
|
|
JOJO-1,
JO-PRO-1 |
529 |
545 |
101,
104,
105 |
|
|
|
|
Lissamine
rhodamine B |
570 |
590 |
102 |
|
40 |
41 |
|
LOLO-1,
LO-PRO-1 |
565 |
579 |
|
|
|
39 |
|
Lucifer Yellow |
428 |
536 |
|
15 |
|
14 |
|
LysoSensor
Blue (pH 5) |
374 |
424 |
129 |
|
05 |
06,
10 |
|
LysoSensor
Green (pH 5) |
442 |
505 |
|
15 |
77 |
14,71 |
|
LysoSensor
Yellow/Blue (pH 4.2) |
384 |
540 |
129 |
|
13 |
|
04,
07 |
LysoTracker®
Green |
504 |
511 |
104,
105 |
25 |
|
22,
23 |
|
LysoTracker®
Red |
577 |
592 |
102 |
|
40 |
41 |
|
LysoTracker®
Yellow |
534 |
551 |
105 |
30,
34 |
|
32 |
|
Mag-Fura-2 |
330 |
491 |
|
|
|
|
04 |
Mag-Indo-1 |
330 |
417 |
|
|
|
|
07 |
Magnesium Green |
506 |
531 |
104,
105 |
|
|
|
|
Marina Blue® |
365 |
460 |
|
|
02,
05 |
03,
06 |
|
4-Methylumbelliferone |
360 |
449 |
|
|
02,
05 |
03,
06 |
|
Mithramycin |
395 |
535 |
|
15 |
|
14 |
|
MitoTracker®
Green |
490 |
516 |
100,
115 |
25 |
|
22,
23 |
|
MitoTracker®
Orange |
551 |
576 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
MitoTracker®
Red |
578 |
599 |
102 |
|
40 |
41 |
|
NBD (amine) |
465 |
535 |
|
|
18,
19 |
|
|
Nile Red |
549 |
628 |
35,
39,
103 |
34,
38 |
|
|
|
Oregon
Green® 488 |
496 |
524 |
100,
115 |
25 |
|
22,
23 |
|
Oregon
Green® 500 |
503 |
522 |
104,
105 |
|
|
|
|
Oregon
Green® 514 |
511 |
530 |
104,
105 |
|
|
|
|
Pacific Blue |
410 |
455 |
119 |
|
|
|
|
PBF1 |
334 |
504 |
|
|
|
|
04 |
C-phycocyanin |
620 |
648 |
|
|
|
44,
45,
46 |
|
R-phycocyanin |
618 |
642 |
102 |
|
|
43,
44 |
|
R-phycoerythrin
(PE) |
565 |
575 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
PKH26 |
551 |
567 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
POPO-1,
PO-PRO-1 |
434 |
456 |
114,
130 |
|
|
|
|
POPO-3,
PO-PRO-3 |
534 |
572 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Propidium Iodide |
536 |
617 |
35,
103 |
34 |
|
|
|
PyMPO |
415 |
570 |
|
15 |
|
|
|
Pyrene |
345 |
378 |
|
|
02 |
|
|
Pyronin Y |
555 |
580 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Quinacrine Mustard |
423 |
503 |
|
15 |
|
14 |
|
Resorufin |
570 |
585 |
102 |
|
|
37,
39 |
|
Red
Fluorescent Protein (DsRed) |
561 |
585 |
111 |
|
|
|
|
RH 414 |
500 |
635 |
103 |
25,
30 |
|
21 |
|
Rhod-2 |
550 |
571 |
101,
108 |
|
|
|
|
Rhodamine B |
555 |
580 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Rhodamine Green |
502 |
527 |
104,
105 |
|
|
|
|
Rhodamine Red |
570 |
590 |
102 |
|
40 |
39,
41 |
|
Rhodamine Phalloidin |
542 |
565 |
101,
108 |
34,
38 |
|
37 |
|
Rhodamine 110 |
496 |
520 |
100,
115 |
25 |
|
22,
23 |
|
Rhodamine 123 |
507 |
529 |
104,
105 |
|
|
|
|
5-ROX (carboxy-X-rhodamine) |
574 |
602 |
102 |
|
40 |
39,
41 |
|
SBFI |
334 |
525 |
|
|
|
|
04 |
SITS |
337 |
436 |
|
|
02 |
03 |
|
SNAFL®-1
(high pH) |
576 |
635 |
|
|
|
|
29 |
SNAFL®-2 |
525 |
546 |
|
|
|
|
29 |
SNARF®-1
(high pH) |
575 |
635 |
|
30 |
|
|
31,
72 |
Sodium Green |
507 |
535 |
104,
105 |
|
|
|
|
SpectrumAqua® |
433 |
480 |
114,
130 |
|
13 |
11 |
|
SpectrumGreen®
#1 |
497 |
524 |
100,
115 |
25 |
|
22,
23 |
|
SpectrumGreen®
#2 |
509 |
538 |
104,
105 |
30 |
|
|
|
SpectrumOrange® |
559 |
588 |
101,
108 |
34,
38 |
|
32,
33,
37,39 |
|
SpectrumRed® |
587 |
612 |
102 |
|
40 |
41,
42 |
|
SYTO® 11 |
508 |
527 |
104,
105 |
|
|
|
|
SYTO® 13 |
488 |
509 |
100,
115 |
25 |
|
22,
23 |
|
SYTO® 17 |
621 |
634 |
|
|
|
42,
43 |
|
SYTO® 45 |
452 |
484 |
114,
130 |
|
|
|
|
SYTOX® Blue |
445 |
470 |
114,
130 |
|
13 |
|
|
SYTOX® Green |
504 |
523 |
104,
105 |
|
|
|
|
SYTOX® Orange |
547 |
570 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
5-TAMRA (5-Carboxytetramethylrhodamine) |
542 |
568 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Tertramethylrhodamine
(TRITC) |
555 |
580 |
101,
108 |
34,
38 |
|
32,
33,
37 |
|
Texas
Red®/Texas Red®-X |
595 |
615 |
102 |
|
40 |
42,
43 |
|
Thiadicarbocyanine |
651 |
671 |
|
|
|
46,
47 |
|
TOTO®-1/
TO-PRO®-1 |
514 |
533 |
104,
105 |
30 |
|
|
|
TOTO®-3,
TO-PRO®-3 |
642 |
660 |
100 |
|
|
45,
46,
47 |
|
TO-PRO®-5 |
748 |
768 |
143 |
|
|
|
|
WW 781 |
605 |
639 |
|
|
|
45 |
|
X-Rhodamine (XRITC) |
580 |
605 |
102 |
|
40 |
39,
41 |
|
YFP (Yellow
Fluorescent Protein) |
513 |
527 |
104,
105 |
|
|
|
|
YOYO®-1,
YO-PRO®-1 |
491 |
509 |
100,
115 |
25 |
|
22,
23 |
|
YOYO®-3,
YO-PRO®-3 |
612 |
631 |
102 |
|
|
43 |
|
|