Introduction to the Filters for Fluorescence Microscopy Guide

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Filters for Fluorescence

Index

Introduction

Choosing the Right Filter Set

Fluorophore Reference Table

Filter Sets

Exciters

Dichroics

Emitters

Filter Holders

FAQs

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Light Sources & Detectors

Emission Colour Chart

Neutral Density Filters

FRET

Accessory Filters

Mouse Kidney, images using Fluorescence Filter Sets Glen Spectra has produceded this guide to make your filter selection easier and quicker.

You can search for the fluorophore you wish to use and we will match the filter set for you!

Alternatively, you can look at our filter sets and their spectra and decide which one would be most suitable for you.

If you don't require a full filter set, why not look at our range of separate filter components: exciters, dichroics and emitters. You can then mix and match to your requirement.

Have a question? See our FAQs page for the answer. If the information you need isn't there, contact and we would be pleased to help.

Pioneering Fluorescence

We specialise in the design and manufacture of high performance, optical interference filters for fluorescence microscopy. We offer an extensive selection of dye-specific filter sets for single and multi-label microscopy applications, including DsRed, GFP, Pinkel, Ratio-Imaging, FRET, and other techniques.

We have pioneered developments in filter technology for fluorescence microscopy. Over the years, innovation in product design focused on improving throughput and polarisation, while minimising auto-fluorescence and cross-talk. The resulting optimised filter sets for epi-luminescent microscopes have enhanced the ability of life scientists to isolate small Stokes-shifted energy of low intensity and image complex cellular structures and processes.

From the beginning, our filter technology inventions has stimulated the science of optical microscopy and fluorescence detection. We remain committed to pushing the limits of the science through collaboration with researchers and manufacturers worldwide.

General Features

All our fluorescence microscopy filter sets are optimised to maximise emission collection and minimise optical degradation by using specialised optical polishing and anti-reflective coatings. Exciters are high performance bandpass filters with reflective metal layers tolerant to intense excitation sources. Dichroics are hard surface coated. Emitters are anti-reflection coated to minimise back reflection and enhance transmission typically to 90%.