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Detailed below are the
filter sets that utilise Alpha Technology for the emission filters.
This results in much steeper edges than any other filter
manufacturing method, while increasing attenuation and virtually
eliminating auto-fluorescence. Excitation filters use a bandpass
filter design as in Vivid Class and Vivid Standard sets. Dichroics
are hard surface coated. Emission fiters are ALPHA longpass designs.
Vivid Plus sets provide the brightest images for most fluorescent
dyes, with less colour definition than the ALPHA Vivid Sets.
Click on any of the part
numbers to view a spectrum.
Part
Number |
Fluorophores |
Application |
Components |
Type |
Part
Number |
Description |
XF02-2 |
DAPI
Hoechst
33342 & 33258
AMCA/AMCA-X |
Wide
band exciter |
Exciter
Dichroic
Emitter |
XF1001
XF2001
XF3097 |
330WB80
400DCLP
400ALP |
XF05-2 |
DAPI
Hoechst
33342 & 33258
AMCA/AMCA-X |
Good
with mercury arc lamp |
Exciter
Dichroic
Emitter |
XF1005
XF2001
XF3097 |
365WB50
400DCLP
400ALP |
XF13-2 |
Cascade
Blue®
Cascade
Yellow
SpectrumAqua®
SYTOX®
Blue |
- |
Exciter
Dichroic
Emitter |
XF1008
XF2040
XF3091 |
405DF40
445DRLP
450ALP |
XF18-2 |
Fura
Red (high calcium)
DiA
(4-Di-16-ASP) |
Broad
exciter |
Exciter
Dichroic
Emitter |
XF1012
XF2008
XF3093 |
455DF70
515DRLP
515ALP |
XF19-2 |
Acridine
orange (+RNA)
Auramine
O-Feulgen |
- |
Exciter
Dichroic
Emitter |
XF1013
XF2008
XF3093 |
470DF35
515DRLP
515ALP |
XF40-2 |
Texas
Red®/Texas Red®-X
Cy3.5®
MitoTracker®
Red
SNARF®-1
(high pH) |
- |
Exciter
Dichroic
Emitter |
XF1022
XF2019
XF3094 |
560DF40
590DRLP
600ALP |
XF48-2 |
Cy5.5® |
Non-visual
detection |
Exciter
Dichroic
Emitter |
XF1028
XF2024
XF3095 |
670DF20
690DRLP
700ALP |
XF77-2 |
FITC
BODIPY®
FL
Alexa
Fluor® 488 |
- |
Exciter
Dichroic
Emitter |
XF1031
XF2027
XF3092 |
450DF55
485DRLP
500ALP |
XF106-2 |
CCF2
(GeneBLAzer) |
Combines
blue and green |
Exciter
Dichroic
Emitter |
XF1006
XF2005
XF3088 |
400DF15
420DCLP
435ALP |
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