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Fluorescence Resonance
Energy Transfer (FRET) occurs when the emission of one fluorophore
overlaps the absorbance of a second fluorophore and is proportional
to their intermolecular distance (for review, see Wu and Brand,
Analytical Biochem, 218, 1-13, 1994). If the donor fluorophore is
excited with the appropriate wavelength of light, the result is
quenching of its emission and indirect excitation and emission from
the acceptor fluorophore.
Developed in the
laboratory of Dr. Roger Tsien at UCSD (Miyawaki et al, Nature, 388,
882-887, 1977), FRET using GFP spectral mutants provides the ability
to localize and monitor non-binding and molecular protein-protein
interaction in living cells.
Glen Spectra Ltd offer filter
sets for ratiometric measurement of CFP/YFP or BFP/GFP FRET emission.
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