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3rd Millennium Filter Sets

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Filters for Fluorescence

Index

Introduction

Choosing the Right Filter Set

Fluorophore Reference Table

Filter Sets

Exciters

Dichroics

Emitters

Filter Holders

FAQs

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Light Sources & Detectors

Emission Colour Chart

Neutral Density Filters

Accessory Filters

The latest technological advance; 3rd Millennium filters combine a patented, hermetic physical assembly with proprietary ALPHA Technology. A process which produces exceptionally steep cut-on and cut-off slopes. This results in more precise location of cut-on and cut-off points, the ability to place transmission and rejection regions extremely close together, and higher attenuation between the pass band and the rejection band. the physical assembly eliminates polymer adhesives, minimising the potential for photodamage and thermal instability. The result is longer filter life, increased transmission, reduce parasitic fluorescence and significantly reduced Transmitted Wavefront Distortion(TWD)

These filters sets offer the highest possible performance and are available as stock items for seven common Fluorophores:

Set Part Number

Fluorophores

Components

Type

Part Number

Description

3RD-F1

DAPI

Exciter
Dichroic
Emitter
IR Blocker

XF1005
XF2000
3RD/434-485
3RD/B10

365WB50
400DCLP
434-485BP

3RD-F2

CFP

Exciter
Dichroic
Emitter
IR Blocker

3RD/401-451
XF2034
3RD/471-500
3RD/B10

470AF50
500DRLP
471-500BP

3RD-F3

FITC
eGFP

Exciter
Dichroic
Emitter
IR Blocker

3RD/455-504
XF2010
3RD/510-560
3RD/B10

455-504BP
505DRLP
510-560BP

3RD-F4

YFP

Exciter
Dichroic
Emitter
IR Blocker

3RD/485-515
XF2030
3RD/525-560
3RD/B10

485-515BP
525DRLP
525-560BP

3RD-F5

TRITC

Exciter
Dichroic
Emitter
IR Blocker

3RD/505-555
XF2017
3RD/570-650
3RD/B11

5505-555BP
560DRLP
570-650BP

3RD-F6

Texas Red®

Exciter
Dichroic
Emitter
IR Blocker

3RD/535-590
XF2029
3RD/607-695
3RD/B11

535-590BP
595DRLP
607-695BP

3RD-F7

Cy5®

Exciter
Dichroic
Emitter
IR Blocker

3RD/570-645
XF2072
3RD/660-730
IR Blocker

570-645BP
650DRLP
660-730BP

Microscope Compatibility :
25mm diameter standard ring fits the following microscopes:
Nikon: Eclipse
Olympus: IX, & BX
Zeiss: Axio sliders, Turret, & Plus

Filter Specifications :
Assembly Diameter: 25mm
Assembly Thickness: 5.5mm

Substrates: SiO2 substrate with a broadband anti-reflection coating

Note on IR Blocker:
An additional filter may be required to extend attenuation to the limits of the detector (1150nm for silicon) & to reduce the heat load on the optical system. Many lamp housings & detectors have existing filters to remove long wavelength energy. For optimal performance we recommend locating the auxiliary attenuation/heat filter (when required) as close to the detector as possible, except in situations where another component of the optical system is more susceptible to heat.

Standard Blocker Sizes: 18mm, 25mm, & 32mm. Please specify size when ordering. Custom sizes are available.

Fluorophore Reference Table

Filter Sets

Exciters

Dichroics

Emitters

Filter Holders

Neutral Density Filters

Accessory Filters

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